Journal: PLoS ONE
Article Title: The Differential Proliferative Ability of Satellite Cells in Lantang and Landrace Pigs
doi: 10.1371/journal.pone.0032537
Figure Lengend Snippet: Electrophoresed protein was transferred to a polyvinylidene difluoride membrane by electrophoretic blotting. The membrane was incubated with primary antibodies for (A) MyoD, (B) Myogenin, (C) Myostatin, (D) p-mTOR, (E) p-eIF4E, (F) S6K with horseradish peroxidase- labeled anti-mouse, anti-goat and anti-rabbit IgG. Proteins were visualized with the ECL-Plus Western Blotting Reagent (Amersham Pharmacia Biotech, Piscataway, NJ) and exposed to Kodak X-Omat film. The density of the bands was analyzed using Image Analysis Software (Tanon, China). The results are representative of 3 separate experiments. Bars are the mean ± SEM. * Indicates a significant difference ( P <0.05).
Article Snippet: The membranes were incubated overnight at 4°C with 1∶5000 rabbit monoclonal antibody against eIF4E phospho(pS209) (Epitomics, Inc., California), 1∶250 rabbit polyclonal antibody against S6K (Abcam, Cambridge, UK), 1∶1000 rabbit monoclonal antibody against mTOR/FRAP phospho(pS2448) (Epitomics, Inc., California, US), 1∶600 mouse polyclonal antibody against myostatin (Santa Cruz Biotechnology, Santa Cruz, CA), 1∶800 mouse polyclonal antibody against MyoD (Santa Cruz Biotechnology, Santa Cruz, CA), 1∶800 goat polyclonal antibody against myogenin (Santa Cruz Biotechnology, Santa Cruz, CA), and 1∶1000 mouse monoclonal antibody against β-actin (Santa Cruz Biotechnology, Santa Cruz, CA).
Techniques: Membrane, Incubation, Labeling, Western Blot, Software